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anti cd68 antibody  (Elabscience Biotechnology)


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    Structured Review

    Elabscience Biotechnology anti cd68 antibody
    MMP12 silencing inhibited M2 macrophage polarization. THP-1 cells were differentiated into M0 macrophages (THP-1 M0) by treatment with 100 ng/mL PMA for 24 h. (A) Flow cytometry was used to quantify the number of <t>CD68-positive</t> cells. Subsequently, KYSE150 cells were co-cultured with the THP-1-derived macrophages using a Transwell system. (B) The mRNA levels of IL-10, Arg-1, and TGF-β were detected by qRT-PCR. (C) Flow cytometry was used to quantify the number of CD206-positive macrophages. (D) Cell migration analysis by transwell migration assay. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.
    Anti Cd68 Antibody, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd68+antibodies/pmc13022693-101-14-17?v=Elabscience+Biotechnology
    Average 94 stars, based on 8 article reviews
    anti cd68 antibody - by Bioz Stars, 2026-07
    94/100 stars

    Images

    1) Product Images from "WTAP stabilizes MMP12 expression to promote the malignant phenotypes of esophageal cancer cells"

    Article Title: WTAP stabilizes MMP12 expression to promote the malignant phenotypes of esophageal cancer cells

    Journal: Regenerative Therapy

    doi: 10.1016/j.reth.2026.101101

    MMP12 silencing inhibited M2 macrophage polarization. THP-1 cells were differentiated into M0 macrophages (THP-1 M0) by treatment with 100 ng/mL PMA for 24 h. (A) Flow cytometry was used to quantify the number of CD68-positive cells. Subsequently, KYSE150 cells were co-cultured with the THP-1-derived macrophages using a Transwell system. (B) The mRNA levels of IL-10, Arg-1, and TGF-β were detected by qRT-PCR. (C) Flow cytometry was used to quantify the number of CD206-positive macrophages. (D) Cell migration analysis by transwell migration assay. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.
    Figure Legend Snippet: MMP12 silencing inhibited M2 macrophage polarization. THP-1 cells were differentiated into M0 macrophages (THP-1 M0) by treatment with 100 ng/mL PMA for 24 h. (A) Flow cytometry was used to quantify the number of CD68-positive cells. Subsequently, KYSE150 cells were co-cultured with the THP-1-derived macrophages using a Transwell system. (B) The mRNA levels of IL-10, Arg-1, and TGF-β were detected by qRT-PCR. (C) Flow cytometry was used to quantify the number of CD206-positive macrophages. (D) Cell migration analysis by transwell migration assay. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

    Techniques Used: Flow Cytometry, Cell Culture, Derivative Assay, Quantitative RT-PCR, Migration, Transwell Migration Assay



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    MMP12 silencing inhibited M2 macrophage polarization. THP-1 cells were differentiated into M0 macrophages (THP-1 M0) by treatment with 100 ng/mL PMA for 24 h. (A) Flow cytometry was used to quantify the number of <t>CD68-positive</t> cells. Subsequently, KYSE150 cells were co-cultured with the THP-1-derived macrophages using a Transwell system. (B) The mRNA levels of IL-10, Arg-1, and TGF-β were detected by qRT-PCR. (C) Flow cytometry was used to quantify the number of CD206-positive macrophages. (D) Cell migration analysis by transwell migration assay. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.
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    Image Search Results


    MMP12 silencing inhibited M2 macrophage polarization. THP-1 cells were differentiated into M0 macrophages (THP-1 M0) by treatment with 100 ng/mL PMA for 24 h. (A) Flow cytometry was used to quantify the number of CD68-positive cells. Subsequently, KYSE150 cells were co-cultured with the THP-1-derived macrophages using a Transwell system. (B) The mRNA levels of IL-10, Arg-1, and TGF-β were detected by qRT-PCR. (C) Flow cytometry was used to quantify the number of CD206-positive macrophages. (D) Cell migration analysis by transwell migration assay. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

    Journal: Regenerative Therapy

    Article Title: WTAP stabilizes MMP12 expression to promote the malignant phenotypes of esophageal cancer cells

    doi: 10.1016/j.reth.2026.101101

    Figure Lengend Snippet: MMP12 silencing inhibited M2 macrophage polarization. THP-1 cells were differentiated into M0 macrophages (THP-1 M0) by treatment with 100 ng/mL PMA for 24 h. (A) Flow cytometry was used to quantify the number of CD68-positive cells. Subsequently, KYSE150 cells were co-cultured with the THP-1-derived macrophages using a Transwell system. (B) The mRNA levels of IL-10, Arg-1, and TGF-β were detected by qRT-PCR. (C) Flow cytometry was used to quantify the number of CD206-positive macrophages. (D) Cell migration analysis by transwell migration assay. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

    Article Snippet: For immunophenotyping, single-cell suspensions of both THP-1 and THP-1-M0 cells were labeled with an anti-CD68 antibody (E-AB-F1299L, Elabscience, Wuhan, China).

    Techniques: Flow Cytometry, Cell Culture, Derivative Assay, Quantitative RT-PCR, Migration, Transwell Migration Assay

    Immunohistochemical staining for immune cell markers in the epithelial + superficial connective tissue region. Representative immunohistochemical images of the superficial peri-implant soft tissue zone stained for MPO, CD68, CD3, CD20, and CD138. Scale bars: 100 μm. Red dashed boxes and red arrows indicate the magnified regions (insets).

    Journal: International Dental Journal

    Article Title: Immunological Features of Peri-Implant Soft Tissue After Healing Abutment Dislodgement: A Comparative Human Study

    doi: 10.1016/j.identj.2026.109521

    Figure Lengend Snippet: Immunohistochemical staining for immune cell markers in the epithelial + superficial connective tissue region. Representative immunohistochemical images of the superficial peri-implant soft tissue zone stained for MPO, CD68, CD3, CD20, and CD138. Scale bars: 100 μm. Red dashed boxes and red arrows indicate the magnified regions (insets).

    Article Snippet: Sections were then incubated overnight at 4 °C with primary antibodies (MPO, CD68, CD3, CD20 and CD138, all purchased from Boster, China; dilution 1:400).

    Techniques: Immunohistochemical staining, Staining